About Us > Facilities

The CDR has available 11,000 square feet of new space designed and equipped for studies on the biochemistry, molecular biology and morphology of the CNS, consisting of 6 laboratories, Electron Microscope (EM) and confocal microscopy facilities, darkrooms, 3 walk-in cold rooms, 4 tissue culture facilities, a glassware washing unit, segregated area for radioisotope studies, and tissue processing and histology facilities.  The Geriatric Psychiatry Research Division includes 15 rooms.  These include 2 separate examination rooms fully equipped with exam tables, ECG machines, and centrifuges for processing of biological samples.  It also has 2 separate offices for neuropsychological assessment; 1 equipped with postural sway apparatus.

Electron Microscopy (EM) Facility

Headed by Dr. Chris Goulbourne, the electron microscopy (EM) facility is a core facility that provides EM service to investigators. The facility houses an ThermoFisher Talos L120C TEM that has the capability to do 2D imaging, dual axis electron tomography for 3D ultrastructural evaluation and cryo EM. Services offered include ultrastructural evaluation in 2D and 3D, protein localization using immunogold techniques and cryogenic evaluation of proteins, viruses and cellular architecture. Additionally, the facility offers advice and sample preparation for employing correlative light and electron microcopy(CLEM) and Nano Secondary Ion Mass spectrometry (NanoSIMS) for tracking stable isotope tagged molecules. Sample preparation instrumentation includes a Leica UCT ultramicrotome and ThermoFisher Mark IV Vitrobot. We have a dedicated high performance work station for processing 3D data sets using Amira and Imod software. The services of the facility are currently available to external academic institutes and industrial facilities on a fee-for-use-basis. Further information can be obtained by contacting Dr. Chris Goulbourne at Chris.Goulbourne@NKI.rfmh.org

The FEI Talos L120C TEM has magnification between 25X upto 650,000x and 0.2nm line resolution. Capabilties include electron tomography for 3D ultrastructural evaluation, regular 2D imaging and cryoelectron microscopy.
The FEI Vitrobot Mark IV is used for cryo sample preparation of cells, exosomes, proteins and viruses. The Leica Reichert Ultracut S microtome with Diatome diamond knife for sectioning samples between 60 to 1000nm.
The PELCO easiGlow is used to glow discharge grids to make them hydrophilic for even sample distribution. The LKB knife maker is used to make glass knives for preparing the sample block face. Dedicated high performance computer workstation for processing and visualizing tomographic data sets. Used for visualising cellular ultrastructure in 3D.

TEM Images

Cryo electron microscopy of extracellular vesicles isolated from a 12-month-old mouse brain. Magnification 45000x. Levy Laboratory Mouse frontal cortex ultrastructure. Magnification 5300x. Nixon Laboratory Neuron and glia nuclei that have been immunogold labeled using an anti histone antibody that has been silver enhanced. Magnification 4300X. Nixon Laboratory
Cryo electron microscopy of Down Syndrome Fibroblast organelles. Magnification 22000X. Nixon Laboratory Serial Block Face Scanning Electron Microscopy 3D reconstruction of 50µm thick mouse cortex using a ThermoFisher Apreo Volumescope. Nixon Laboratory

External Institute TEM Images

Negative stain of Bacteriophage, Magnification 92000x, Dominican College, NY Lung tissue and Alveolar Type II Cell, Magnification 2600x, UCLA, CA Longitudinal section of mouse skeletal muscle, Magnification 5300x, Icahn Sinai School of Medicine at Mount Sinai, NY
Mitochondria in cardiac tissue. Magnification 13500X. Columbia University, NY Immuno EM of pathological Tau present at the synapse. Magnification 57000X Columbia University, NY and University College London, UK Differentiated alpha cell in skeletal muscle. Magnification 3400X, Columbia University, NY

TEM Publications

  • Peng KY, Pérez-González R, Alldred MJ, Goulbourne CN, Morales-Corraliza J, Saito M, Saito M, Ginsberg SD, Mathews PM, Levy E. Apolipoprotein E4 genotype compromises brain exosome production. Brain 2018; 142(1):163-175. Click for abstract
  • D'Acunzo P, Hargash T, Pawlik M, Goulbourne CN, Pérez-González R, Levy E. Enhanced Generation of Intraluminal Vesicles in Neuronal Late Endosomes in the Brain of a Down Syndrome Mouse Model with Endosomal Dysfunction. Dev Neurobiol 2019; 79(7): 656-663. Click for abstract
  • Mathews PM, Levy E. Exosome Production Is Key to Neuronal Endosomal Pathway Integrity in Neurodegenerative Diseases. Front Neurosci 2019; 13:1347. Click for abstract
  • Pensalfini A, Kim S, Subbanna S, Bleiwas C, Goulbourne CN, Stavrides PH, Jiang Y, Lee J-H, Darji S, Pawlik M, Huo C, Peddy J, Berg MJ, Smiley JF, Basavarajappa BS, Nixon RA. Endosomal Dysfunction induced by directly overactivating Rab5 recapitulates prodromal and neurodegenerative features of Alzheimer’s disease. Cell Rep 2020 Nov24:33(8):108420 PMID: 33238112 Click for abstract
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  • D'Acunzo P, Pérez-González R, Kim Y, Hargash T, Miller C, Alldred MJ, Erdjument-Bromage H, Penikalapati SC, Pawlik M, Saito M, Saito M, Ginsberg SD, Neubert TA, Goulbourne CN, Levy E. Sci Adv. 2021 Feb 12;7(7):eabe5085. doi: 10.1126/sciadv.abe5085 PMID:33579698 Click for abstract
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  • Zhang Y, Varela L, Szigeti-Buck K, Williams A, Stoiljkovic M, Šestan-Peša M, Henao-Mejia J, D'Acunzo P, Levy E, Flavell RA, Horvath TL, Kaczmarek LK. Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1. Nat Commun. 2021 Mar 19;12(1):1731. doi: 10.1038/s41467-021-22003-8. PMID: 33741962 Click for abstract
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  • Lie PPY, Yang DS, Stavrides P, Goulbourne CN, Zheng P, Mohan PS, Cataldo AM, Nixon RA. Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons. Cell Rep 2021 35, 109034. doi:10.1016/j.celrep.2021.109034 Click for abstract
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NKI Health Sciences Library

Under library director, Stuart Moss, MLS, the Health Sciences Library of the Nathan Kline Institute offers an extensive array of mental health and biomedical information resources in both print and electronic formats. The research-oriented collection is particularly strong in psychiatry, neuroscience, and psychopharmacology. It includes over 300 current journal subscriptions, more than 25,000 bound journal volumes and books, as well as access to the entire MEDLINE and PsycINFO databases, and toNOVEL, the New York Online Virtual Electronic Library. The NKI Library is one of the best sources for psychiatric information in the Hudson valley region.

Center for Biomedical Imaging and Neuromodulation (C-BIN)

Known (formerly CABI) has yielded the first images of the brain in transgenic mice modeling the pathology of Alzheimer’s disease. The sensitive detection of pathology in animal models has MRIprovided the basis for new neuro-imaging approaches to characterize patients with mild cognitive impairment or AD. The approximately 12,000 sq. ft. facility is centered around the technology of Magnetic Resonance Imaging (MRI) and Magnetic Resonance Spectroscopy (MRS) and houses three state-of-the-art MR systems. Headed by Michael Milham, MD, PhD, C-BIN is divided into the Computational Neuroimaging Laboratory; Design, Acquisition and Stimulation Laboratory (DASL); and Medical Physics Laboratory (MPL). These subdivisions work together to understand the impact of psychiatric illnesses on developmental processes utilizing innovative MRI-based imaging approaches to characterize brain maturation across the lifespan.

Genomics Facility

The Genomics Facility at the Nathan Kline Institute has state-of-the-art instrumentation for molecular and cellular based operations, including single population cellular analyses. Dr. Ginsberg oversees the instrumentation that includes a new laser capture microdissection (LCM) system (Leica LMD 7000) which enables precise microaspiration of neurons and other cells fromLMC tissue sections. This instrumentation is critical for analyses at the NKI of vulnerable and relatively spared populations of neurons in neurodegenerative disorders including Alzheimer's disease (AD) and Down syndrome (DS), among others. Cells can be isolated from a wide variety of cell culture, animal model, and human postmortem brain tissue preparations. The NKI Genomics Facility also houses qPCR instrumentation and robotics for the generation of custom-designed microarray platforms. Please feel free to contact Dr. Ginsberg if you are interested in learning more about the instrumentation or the experiments that are conducted using these tools.